Pharmacological and Toxicological Screening Methods - I

THEORY 1.

Laboratory Animals Common laboratory animals:

Description, handling and applications of different species and strains of animals.

Transgenic animals: Production, maintenance and applications Anaesthesia and euthanasia of experimental animals.

Maintenance and breeding of laboratory animals.

CPCSEA guidelines to conduct experiments on animals Good laboratory practice.

Bioassay-Principle, scope and limitations and methods Hrs Preclinical screening of new substances for the pharmacological activity using in vivo, in vitro, and other possible animal alternative models.

General principles of preclinical screening. CNS Pharmacology:

behavioral and muscle co ordination, CNS stimulants and Hrs depressants, anxiolytics, anti-psychotics, anti epileptics and nootropics.

Drugs for neurodegenerative diseases like Parkinsonism, Alzheimers and multiple sclerosis. Drugs acting on Autonomic Nervous System.

Preclinical screening of new substances for the pharmacological activity using in vivo, in vitro, and other possible animal alternative models.

Respiratory Pharmacology: anti-asthmatics, drugs for COPD and anti allergics.

Reproductive Pharmacology:

Aphrodisiacs and antifertility agents Analgesics, antiinflammatory and antipyretic agents. Gastrointestinal drugs:

anti ulcer, anti -emetic, anti- diarrheal and laxatives.

Hrs Preclinical screening of new substances for the pharmacological activity using in vivo, in vitro, and other possible animal alternative models.

Cardiovascular Pharmacology: antihypertensives, antiarrythmics, antianginal, antiatherosclerotic agents and diuretics. Drugs for metabolic disorders like anti-diabetic, antidyslipidemic agents.

Anti cancer agents. Hepatoprotective screening methods.

Hrs Preclinical screening of new substances for the pharmacological activity using in vivo, in vitro, and other possible animal alternative models.

Iimmunomodulators, Immunosuppressants and immunostimulants General principles of immunoassay:

theoretical basis and optimization of immunoassay, heterogeneous and homogenous immunoassay systems.

Immunoassay methods evaluation; protocol outline, objectives and preparation. Immunoassay for digoxin and insulin Limitations of animal experimentation and alternate animal experiments.

Extrapolation of in vitro data to preclinical and preclinical to humans Hrs