Cellular and Molecular Pharmacology

THEORY 1.

Cell biology Structure and functions of cell and its organelles Genome organization.

Gene expression and its regulation, importance of siRNA and micro RNA, gene mapping and gene sequencing Cell cycles and its regulation.

Cell death– events, regulators, intrinsic and extrinsic pathways of apoptosis.

Necrosis and autophagy.

Cell signaling Intercellular and intracellular signaling pathways.

Classification of receptor family and molecular structure ligand gated ion channels; G-protein coupled receptors, tyrosine kinase receptors and nuclear receptors.

Secondary messengers: cyclic AMP, cyclic GMP, calcium ion, inositol 1,4,5-trisphosphate, (IP3), NO, and diacylglycerol.

Detailed study of following intracellular signaling pathways: cyclic AMP signaling pathway, mitogen-activated protein kinase (MAPK) signaling, Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway.

Hrs Hrs Principles and applications of genomic and proteomic tools DNA electrophoresis, PCR (reverse transcription and real time), Gene sequencing, micro array technique, SDS page, ELISA and western blotting, Recombinant DNA technology and gene therapy Basic principles of recombinant DNA technology-Restriction enzymes, various types of vectors. Applications of recombinant DNA technology.

Gene therapy- Various types of gene transfer techniques, clinical applications and recent advances in gene therapy.

Hrs Pharmacogenomics Gene mapping and cloning of disease gene.

Genetic variation and its role in health/ pharmacology Polymorphisms affecting drug metabolism Genetic variation in drug transporters Genetic variation in G protein coupled receptors Applications of proteomics science:

Genomics, proteomics, metabolomics, functionomics, nutrigenomics Immunotherapeutics Types of immunotherapeutics, humanisation antibody therapy, Immunotherapeutics in clinical practice Hrs a.

Cell culture techniques Basic equipments used in cell culture lab. Cell culture media, various types of cell culture, general procedure for cell cultures; isolation of cells, subculture, cryopreservation, characterization of cells and their application.

Principles and applications of cell viability assays, glucose uptake assay, Calcium influx assays Principles and applications of flow cytometry b.

Biosimilars Hrs