Advanced Spectral Analysis

THEORY 1.

UV and IR spectroscopy:

Wood ward – Fieser rule for 1,3- butadienes, cyclic dienes and α, β-carbonyl compounds and interpretation compounds of enones.

ATR-IR, IR Interpretation of organic compounds.

Hrs NMR spectroscopy:

1-D and 2-D NMR, NOESY and COSY, HECTOR, INADEQUATE techniques, Interpretation of organic compounds.

Hrs Mass Spectroscopy Mass fragmentation and its rules, Fragmentation of important functional groups like alcohols, amines, carbonyl groups and alkanes, Meta stable ions, Mc Lafferty rearrangement, Ring rule, Isotopic peaks, Interpretation of organic compounds.

Hrs Chromatography:

Principle, Instrumentation and Applications of the following :

a) GC-MS b) GC-AAS c) LC-MS d) LC-FTIR e) LC-NMR f) CE- MS g) High Performance Thin Layer chromatography h) Super critical fluid chromatography i) Ion Chromatography j) I-EC (Ion- Exclusion Chromatography) k) Flash chromatography Hrs a). Thermal methods of analysis Introduction, principle, instrumentation and application of DSC, DTA and TGA.

b). Raman Spectroscopy Introduction, Principle, Instrumentation and Applications.

c). Radio immuno assay Biological standardization , bioassay, ELISA, Radioimmuno assay of digitalis and insulin.

Hrs